Molecular Weight:Article Title: Methods and systems for producing nanolipoprotein particles
Article Snippet: A freshly made 10 mM solution of EZ-LINK® Sulfo-NHS-LC-Biotin (Pierce) was prepared according to the manufactures recommendation in ddH2O. .. The following materials and equipments were used: 4-20% Tris-Glycine polyacrylamide gel, 1×Tris-Glycine native running buffer, 2× Native Sample buffer, Native Mark molecular weight marker (Invitrogen); SYPRO® Ruby Stain (Bio-Rad) light sensitive, Aqueous destain solution: 10% Methanol; 7% acetic acid; Fluoroimager with appropriate filter for SYPRO® Ruby stain; Biotinylated positive control protein such as biotinylated-bR; Bovine serum albumin 1 mg/ml solution; PBS-Tween buffer: 1.06 mM KH2PO4; 2.97 mM Na2HPO4; NaCl 1551.72 mM, 0.05% tween-20 (v/v) pH 7.4; 1×PBS buffer, (Gibco): 1.06 mM KH2PO4; 2.97 mM Na2HPO4; NaCl 1551.72 mM, pH 7.4; Cyanine-5-Strepavidin (Rockland) solution (5 μg/mL); Barcoded γ-Aminopropylsilane coated glass slides (GAPS-II; Corning); Robotic arrayer; Hybridization Chamber (Grace Bio-Labs); Blocking buffer: 1 mg/mL BSA in 1×PBS, Wash Buffer: 1×PBS; Laser-based confocal scanner (ScanArray 5000 XL; Perkin-Elmer); UV-visible plate reader (Bio-TEK Synergy HT); 96 well flat bottom UV plate (Corning Costar UV Plate). .. Adsorbent beads (Bio beads, Bio-Rad) were also used to remove the detergent.
Marker:Article Title: Methods and systems for producing nanolipoprotein particles
Article Snippet: A freshly made 10 mM solution of EZ-LINK® Sulfo-NHS-LC-Biotin (Pierce) was prepared according to the manufactures recommendation in ddH2O. .. The following materials and equipments were used: 4-20% Tris-Glycine polyacrylamide gel, 1×Tris-Glycine native running buffer, 2× Native Sample buffer, Native Mark molecular weight marker (Invitrogen); SYPRO® Ruby Stain (Bio-Rad) light sensitive, Aqueous destain solution: 10% Methanol; 7% acetic acid; Fluoroimager with appropriate filter for SYPRO® Ruby stain; Biotinylated positive control protein such as biotinylated-bR; Bovine serum albumin 1 mg/ml solution; PBS-Tween buffer: 1.06 mM KH2PO4; 2.97 mM Na2HPO4; NaCl 1551.72 mM, 0.05% tween-20 (v/v) pH 7.4; 1×PBS buffer, (Gibco): 1.06 mM KH2PO4; 2.97 mM Na2HPO4; NaCl 1551.72 mM, pH 7.4; Cyanine-5-Strepavidin (Rockland) solution (5 μg/mL); Barcoded γ-Aminopropylsilane coated glass slides (GAPS-II; Corning); Robotic arrayer; Hybridization Chamber (Grace Bio-Labs); Blocking buffer: 1 mg/mL BSA in 1×PBS, Wash Buffer: 1×PBS; Laser-based confocal scanner (ScanArray 5000 XL; Perkin-Elmer); UV-visible plate reader (Bio-TEK Synergy HT); 96 well flat bottom UV plate (Corning Costar UV Plate). .. Adsorbent beads (Bio beads, Bio-Rad) were also used to remove the detergent.
Staining:Article Title: Methods and systems for producing nanolipoprotein particles
Article Snippet: A freshly made 10 mM solution of EZ-LINK® Sulfo-NHS-LC-Biotin (Pierce) was prepared according to the manufactures recommendation in ddH2O. .. The following materials and equipments were used: 4-20% Tris-Glycine polyacrylamide gel, 1×Tris-Glycine native running buffer, 2× Native Sample buffer, Native Mark molecular weight marker (Invitrogen); SYPRO® Ruby Stain (Bio-Rad) light sensitive, Aqueous destain solution: 10% Methanol; 7% acetic acid; Fluoroimager with appropriate filter for SYPRO® Ruby stain; Biotinylated positive control protein such as biotinylated-bR; Bovine serum albumin 1 mg/ml solution; PBS-Tween buffer: 1.06 mM KH2PO4; 2.97 mM Na2HPO4; NaCl 1551.72 mM, 0.05% tween-20 (v/v) pH 7.4; 1×PBS buffer, (Gibco): 1.06 mM KH2PO4; 2.97 mM Na2HPO4; NaCl 1551.72 mM, pH 7.4; Cyanine-5-Strepavidin (Rockland) solution (5 μg/mL); Barcoded γ-Aminopropylsilane coated glass slides (GAPS-II; Corning); Robotic arrayer; Hybridization Chamber (Grace Bio-Labs); Blocking buffer: 1 mg/mL BSA in 1×PBS, Wash Buffer: 1×PBS; Laser-based confocal scanner (ScanArray 5000 XL; Perkin-Elmer); UV-visible plate reader (Bio-TEK Synergy HT); 96 well flat bottom UV plate (Corning Costar UV Plate). .. Adsorbent beads (Bio beads, Bio-Rad) were also used to remove the detergent.
Positive Control:Article Title: Methods and systems for producing nanolipoprotein particles
Article Snippet: A freshly made 10 mM solution of EZ-LINK® Sulfo-NHS-LC-Biotin (Pierce) was prepared according to the manufactures recommendation in ddH2O. .. The following materials and equipments were used: 4-20% Tris-Glycine polyacrylamide gel, 1×Tris-Glycine native running buffer, 2× Native Sample buffer, Native Mark molecular weight marker (Invitrogen); SYPRO® Ruby Stain (Bio-Rad) light sensitive, Aqueous destain solution: 10% Methanol; 7% acetic acid; Fluoroimager with appropriate filter for SYPRO® Ruby stain; Biotinylated positive control protein such as biotinylated-bR; Bovine serum albumin 1 mg/ml solution; PBS-Tween buffer: 1.06 mM KH2PO4; 2.97 mM Na2HPO4; NaCl 1551.72 mM, 0.05% tween-20 (v/v) pH 7.4; 1×PBS buffer, (Gibco): 1.06 mM KH2PO4; 2.97 mM Na2HPO4; NaCl 1551.72 mM, pH 7.4; Cyanine-5-Strepavidin (Rockland) solution (5 μg/mL); Barcoded γ-Aminopropylsilane coated glass slides (GAPS-II; Corning); Robotic arrayer; Hybridization Chamber (Grace Bio-Labs); Blocking buffer: 1 mg/mL BSA in 1×PBS, Wash Buffer: 1×PBS; Laser-based confocal scanner (ScanArray 5000 XL; Perkin-Elmer); UV-visible plate reader (Bio-TEK Synergy HT); 96 well flat bottom UV plate (Corning Costar UV Plate). .. Adsorbent beads (Bio beads, Bio-Rad) were also used to remove the detergent.
Hybridization:Article Title: Methods and systems for producing nanolipoprotein particles
Article Snippet: A freshly made 10 mM solution of EZ-LINK® Sulfo-NHS-LC-Biotin (Pierce) was prepared according to the manufactures recommendation in ddH2O. .. The following materials and equipments were used: 4-20% Tris-Glycine polyacrylamide gel, 1×Tris-Glycine native running buffer, 2× Native Sample buffer, Native Mark molecular weight marker (Invitrogen); SYPRO® Ruby Stain (Bio-Rad) light sensitive, Aqueous destain solution: 10% Methanol; 7% acetic acid; Fluoroimager with appropriate filter for SYPRO® Ruby stain; Biotinylated positive control protein such as biotinylated-bR; Bovine serum albumin 1 mg/ml solution; PBS-Tween buffer: 1.06 mM KH2PO4; 2.97 mM Na2HPO4; NaCl 1551.72 mM, 0.05% tween-20 (v/v) pH 7.4; 1×PBS buffer, (Gibco): 1.06 mM KH2PO4; 2.97 mM Na2HPO4; NaCl 1551.72 mM, pH 7.4; Cyanine-5-Strepavidin (Rockland) solution (5 μg/mL); Barcoded γ-Aminopropylsilane coated glass slides (GAPS-II; Corning); Robotic arrayer; Hybridization Chamber (Grace Bio-Labs); Blocking buffer: 1 mg/mL BSA in 1×PBS, Wash Buffer: 1×PBS; Laser-based confocal scanner (ScanArray 5000 XL; Perkin-Elmer); UV-visible plate reader (Bio-TEK Synergy HT); 96 well flat bottom UV plate (Corning Costar UV Plate). .. Adsorbent beads (Bio beads, Bio-Rad) were also used to remove the detergent.
Blocking Assay:Article Title: Methods and systems for producing nanolipoprotein particles
Article Snippet: A freshly made 10 mM solution of EZ-LINK® Sulfo-NHS-LC-Biotin (Pierce) was prepared according to the manufactures recommendation in ddH2O. .. The following materials and equipments were used: 4-20% Tris-Glycine polyacrylamide gel, 1×Tris-Glycine native running buffer, 2× Native Sample buffer, Native Mark molecular weight marker (Invitrogen); SYPRO® Ruby Stain (Bio-Rad) light sensitive, Aqueous destain solution: 10% Methanol; 7% acetic acid; Fluoroimager with appropriate filter for SYPRO® Ruby stain; Biotinylated positive control protein such as biotinylated-bR; Bovine serum albumin 1 mg/ml solution; PBS-Tween buffer: 1.06 mM KH2PO4; 2.97 mM Na2HPO4; NaCl 1551.72 mM, 0.05% tween-20 (v/v) pH 7.4; 1×PBS buffer, (Gibco): 1.06 mM KH2PO4; 2.97 mM Na2HPO4; NaCl 1551.72 mM, pH 7.4; Cyanine-5-Strepavidin (Rockland) solution (5 μg/mL); Barcoded γ-Aminopropylsilane coated glass slides (GAPS-II; Corning); Robotic arrayer; Hybridization Chamber (Grace Bio-Labs); Blocking buffer: 1 mg/mL BSA in 1×PBS, Wash Buffer: 1×PBS; Laser-based confocal scanner (ScanArray 5000 XL; Perkin-Elmer); UV-visible plate reader (Bio-TEK Synergy HT); 96 well flat bottom UV plate (Corning Costar UV Plate). .. Adsorbent beads (Bio beads, Bio-Rad) were also used to remove the detergent.
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